Journal: International Journal of Clinical and Experimental Pathology

Article Title: CXCL5 contributes to the tumorigenicity of cervical cancer and is post-transcriptionally regulated by miR-577

doi:

Figure Lengend Snippet: CXCL5 is up-regulated in cervical cancer tissues and cell lines. IHC staining was performed to analyze CXCL5 positive staining in cervical cancer tissues and para-carcinoma tissues (A: left: magnification 40×; right: magnification 200×) and quantitative analysis (B). Western blot was used to measure CXCL5 protein expression in human normal cervical squamous cell line Ect1/E6E7 and cervical cancer cell lines Hela and SiHa (C and D). *P < 0.05. CC, cervical cancer.

Article Snippet: Human normal cervical squamous cell line Ect1/E6E7 (CHI Scientific, Inc., Maynard, MA, USA) and cervical cancer cell lines Hela and SiHa (ATCC, USA) were maintained in DMEM; (Gibco, USA) containing 10% FBS (Gibco, USA) with 5% CO 2 at 37°C. miR-Con, miR-577 mimics, miR-577-Mut, and overexpressed CXCL5 plasmids were purchased from GenePharm (Shanghai, China) and transfected into Hela and SiHa cells using lipofectamine 2000 (Invitrogen).

Techniques: Immunohistochemistry, Staining, Western Blot, Expressing

Journal: NPJ Biofilms and Microbiomes

Article Title: Extracellular vesicles from vaginal Gardnerella vaginalis and Mobiluncus mulieris contain distinct proteomic cargo and induce inflammatory pathways

doi: 10.1038/s41522-024-00502-y

Figure Lengend Snippet: bEV preparations from ( a ) NYC culture medium (control), ( b ) L. crispatus , ( c ) G. vaginalis , and ( d ) M. mulieris were labeled with rhodamine B isothiocyanate and observed in the cytoplasm of ectocervical (Ect1), endocervical (End1), and vaginal epithelial (VK2) cells after 1, 4, and 24 h of incubation. All scale bars are 20 μm.

Article Snippet: Ectocervical (Ect/E6E7, ATCC CRL-2614), endocervical (End1/E6E7, ATCC CRL-2615), and vaginal (VK2/E6E7, ATCC CRL-2616) human epithelial cell lines (American Type Culture Collection, Manassas, VA) were cultured in keratinocyte-serum-free media (K-SFM) supplemented with 0.1 ng/mL epidermal growth factor and 50 μg/mL bovine pituitary extract (Gibco, Life Technologies), 100 U/mL penicillin, and 100 μg/mL of streptomycin at 37 °C in a 5% CO 2 humidified incubator.

Techniques: Control, Labeling, Incubation

Journal: NPJ Biofilms and Microbiomes

Article Title: Extracellular vesicles from vaginal Gardnerella vaginalis and Mobiluncus mulieris contain distinct proteomic cargo and induce inflammatory pathways

doi: 10.1038/s41522-024-00502-y

Figure Lengend Snippet: IL-8 expression measured by ELISA increases with increasing doses of bEVs from G. vaginalis and M. mulieris , but not L. crispatus and NYC culture medium (control), relative to nontreated ( a ) ectocervical, ( b ) endocervical, and ( c ) vaginal epithelial cells. d Exposure to bacterial EVs does not induce significant changes in lactate dehydrogenase release. 29-plex Luminex array identified a multi-cytokine response to bEVs from G. vaginalis and M. mulieris , but not L. crispatus , expressed as fold-changes relative to NYC culture medium control-treated ( e ) ectocervical, ( f ) endocervical, and ( g ) vaginal epithelial cells ( n = 3 per treatment). Error bars indicate standard deviation and asterisks indicate adjusted p -values < 0.05 (*), < 0.01 (**), < 0.001 (***), and <0 .0001 (****) via one-way ANOVA with Tukey’s correction for multiple comparisons.

Article Snippet: Ectocervical (Ect/E6E7, ATCC CRL-2614), endocervical (End1/E6E7, ATCC CRL-2615), and vaginal (VK2/E6E7, ATCC CRL-2616) human epithelial cell lines (American Type Culture Collection, Manassas, VA) were cultured in keratinocyte-serum-free media (K-SFM) supplemented with 0.1 ng/mL epidermal growth factor and 50 μg/mL bovine pituitary extract (Gibco, Life Technologies), 100 U/mL penicillin, and 100 μg/mL of streptomycin at 37 °C in a 5% CO 2 humidified incubator.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control, Luminex, Standard Deviation